Association of coronary heart disease with two polymorphisms within C reactive protein gene promoter region in ethnic Han Chinese
- VernacularTitle:汉族人群C反应蛋白基因多态性与冠心病和C反应蛋白水平相关性研究
- Author:
Xin ZHAO
;
Liang WENG
;
Yao XIAO
;
Jianping CAI
- Publication Type:Journal Article
- Keywords:
C reactive protein,Coronary heart disease,single nucleotide polymorphism
- From:
Chinese Journal of Practical Internal Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of two single nucleotide polymorphism(SNP)-717A/G and-390C/A/T-within the C reactive protein(CRP)promoter region in ethnic Han Chinese and the association with coronary heart disease(CHD).Methods Altogether positive CHD patients and negative controls were studies in this study.The two target fragments were amplied by PCR.Then the-717A/G polymorphism was screened by restriction fragment length polymorphism(RFLP)and the-390C/A/T polymorphism by Sequence Specific PCR(SSP).At last,the association was studied by proper statistical analysis.Results There was no significant difference in the distribution of-717A/G polymorphism betweenpatients and controls.However,the frequencies of allels in-390C/A/T polymorphismwere significant different between them(P
