The Study of Cytokine Level in Sera and Culture Medium of Peripheral Blood Mononuclear Cells in Patients with Hepatitis B Virus Infection
- VernacularTitle:乙型肝炎病毒感染者血清和外周血单个核细胞培养上清细胞因子测定及临床意义
- Author:
Zuming TANG
;
Shouming WANG
;
Jishan ZHENG
- Publication Type:Journal Article
- Keywords:
Hepatitis B;
IFN-?;
IL-4
- From:
Journal of Medical Research
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate cytokine levels in serum and culture medium of peripheral blood mononuclear cells(PBMC) from patients with hepatitis B virus(HBV)infection.Methods PBMCs isolated from fresh heparinized blood were cultured and stimulated with rHBcAg.After 72h at 37℃ 5% CO2 in air,the culture supernatant was collected.Levels of interferon-gamma(IFN-?) and interleukin(IL)-4 in blood serum in spontaneous and supernatant were measured by enzyme-linked immunosorbent assay(ELISA).Results Serum IFN-? levels in patients with acute self-limited hepatitis B(AH) and chronic hapetiti B(CHB) were significantly higher than those in normal control(NC)(P
