Study of the Expression of the Phospholipase A_2,Interleukin-1? and Tissue Inhibitor of Matrix Metalloproteinase-1 Following Traumatic Brain Injury
- VernacularTitle:磷脂酶A_2、白细胞介素-1?、金属蛋白酶组织抑制物-1在创伤性脑损伤中的表达研究
- Author:
Tingfu LI
;
Lihua WAN
;
Wei ZHANG
;
Dengjun FU
- Publication Type:Journal Article
- Keywords:
Phospholipase A2;
Interleukin-1?;
Tissue inhibitor of matrix metalloproteinase-1;
Brain;
Injury
- From:
Journal of Medical Research
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of the cytosolic phospholipase A2(c-PLA2),interleukin-1 beta(IL-1?),tissue inhibitor of matrix metalloproteinase-1(TMP-1) in rat brain after injury.Methods The model of traumatic brain injury originally described by Feeney was employed.The mRNA was analyzed by RT-PCR.Results The mRNA of cytosolic phospholipase A2 was increased at 2 hour and its peak was at 7 day.At 14 day,the level of cytosolic phosphalipase A2 mRNA was still in high level as compared to the control group.Likely,the enhancement expression of interleukin-1? was seen at the 1 hour and the peak time was from 5 to 8 hour.At 72 hour,it decreased to normal level.The expression of inhibitor of matrix metalloproteinase-1 was increased at the 2 hour,and the highest expression level was seen during 48 to72 hour.It came down to normal level at 14 day after injury.Conclusion The augment of the expression of the cytosolic phospholipase A2,interleukin-1? and tissue inhibitor of matrix metalloproteinase-1 following traumatic brain injury suggested that they participated in the pathogenesis of the traumatic brain injury,and may played roles in this pathophysiological process.
