Determination of Imperatorin and Isoimperatorin in Angelicae Dahuricae from Different Sources by HPLC
- VernacularTitle:HPLC测定不同产地白芷饮片中欧前胡素和异欧前胡素的含量
- Author:
Huajie HU
;
Zhaohuan LOU
;
Guiyuan LV
- Publication Type:Journal Article
- Keywords:
Angelica dahurica;
imperatorin;
isoimperatorin;
HPLC
- From:
Journal of Zhejiang Chinese Medical University
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To determine the contents of imperatorin and isoimperation in Angelicae dahuricae from different sources by HPLC,and prove some scientific evidence for processing standardization and quality control of Angelicae dahuricae decoction pieces.[Methods] An HPLC method was established.XB-C18 column was used,with mixture of acetonitrile and water in a gradient mode.The flow rate was 1.0mL?min-1.[Result] The calibration curve of imperatorin was in good linear over the range of 0.0552～0.276?g with the average assay recovery 99.5%.The calibration curve of isoimperatorin was linear over the range of 0.048～0.24?g with the average assay recovery 98.6% (RSD=1.7%).The contents of imperatorin from different sources in Angelicae dahuricae were between 0.046%～0.145%,isoimperatorin was between 0.038%～0.087%,the total was between 0.084%～0.232%.[Conclusion] This method was proved to be rapid,reproducible and accurate.It is suitable for the quality control of Angelilae dahuricae and its preparation.
