Determination of Emodin and Chrysophanol in Rhubarb Sodium Bicarbonate Table by HPLC
- VernacularTitle:HPLC法测定大黄碳酸氢钠片中的大黄素和大黄酚的含量
- Author:
Jinhong CAO
;
Weiqiao PAN
;
Li LIN
- Publication Type:Journal Article
- Keywords:
HPLC;
rhubarb sodium bicarbonate table;
emodin;
chrysophanol
- From:
Journal of Zhejiang Chinese Medical University
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] TO establish an HPLC method for the determination of emodin and chrysophanol in rhubarb sodium bicarbonate tablets.[Methods] An HPLC method was applied;Chromatographic column was Shim-pack clc-ODS(150?6.0mm,5.0um);The mobile phase was methanol-0.1% phosphoric acid (85:15);Velocity of flow was 1.0ml/min;The detaction wavelength was 254nm.[Resulet] The linearty of this method was well.Emodin and chrysophanol average recovery rates were 98.5%,99.3%;RSD=2.75%,2.83%(n=9).[Conclusion] This method is simple,accurate with strong reproducibility,and can be used for the quality control of rhubarb sodium bicarbonate tablets.
