Effect of simulated altitude hypoxia on the activity of matrix metalloproteinase-2 in gingival tissue of chronic periodontitis rat model
- VernacularTitle:模拟高原缺氧对慢性牙周炎大鼠牙龈组织中基质金属蛋白酶-2活性的影响
- Author:
Yao KONG
;
Luchuan LIU
;
Xia ZHOU
- Publication Type:Journal Article
- Keywords:
anoxia;
matrix metalloproteinase 2;
periodontitis
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes in activity of matrix metalloproteinase-2 (MMP-2) in gingival tissue of a chronic periodontitis rat model in normoxia and hypoxia condition, and explore the role of MMP-2 in the development of high altitude periodontal diseases. Methods Thirty-two adult SD rats were randomly divided into 4 groups (8 each): normal control group (N group), normoxia periodontitis group (P1 group), hypoxia control group (H group) and hypoxia periodontitis group (P2 group). The chronic periodontitis rat model was reproduced in P1 and P2 group, and the rats were kept either in normoxia condition or hypoxia condition in a hypobaric chamber in which a condition simulating 5000 meter high altitude was established. Rats without periodontitis in N and H group were treated with the same conditions as control. The activation of MMP-2 in gingival tissue of rats in each group was measured by gelatin zymographic analysis. Results The grey values of activated MMP-2 in N, P1, H and P2 group were 13.72?6.22, 37.23?4.40, 14.45?6.51 and 45.44?4.79, respectively. The value was higher in H group than in N group, in P2 group than in P1 group (P
