Content determination of Podophyllotoxin in two preparations
- VernacularTitle:两种鬼臼毒素制剂中鬼臼毒素含量的测定
- Author:
Xiaohong JIANG
;
Huan HUANG
;
Xiong HUANG
;
Chensheng JIN
- Publication Type:Journal Article
- Keywords:
HPLC;
Podophyllotoxi;
Youdi Liniment;
Content determination
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a HPLC method for determination of Podophyllotoxin in two preparations.Methods:HPLC was carried out,using a Dikma diamonsil C18 column(4.6mm?150mm,5?m)with a column temperature at 25℃,the methanol-water as mobile phase(using gradient)with flowing rate 1.0 mL/min.The detection wavelength was at 292 nm.Results:The method had good linear relationship within the range of 11.8-590?g/mL for Podophyllotoxin,with the correlation coefficients 0.9999.The results of the average recovery of Youdi liniment and Podophyllotoxin tincture were 99.38%,99.62%.Conclusion:The rapid,accurate and reproducible RP-HPLC method was useful for quantification of Podophyllotoxin in Youdi Liniment and Podophyllotoxin Tincture.