Fingerprints establishment of Rhizoma Atractylodis Macrocephalae by high performance liquid chromatography and analysis of plant resource
- VernacularTitle:白术高效液相色谱指纹图谱的建立及种源差异分析
- Author:
Dan SHOU
;
Zhongming YU
;
Jianmin ZHANG
- Publication Type:Journal Article
- Keywords:
Rhizoma Atractylodis macrocephalae;
High Performance Liquid Chromatography(HPLC);
Fingerprint
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the fingerprints of Rhizoma Atractylodis macrocephalae from different origins with high performance liquid chromatography(HPLC)technology,which was furthermore applied in the quality comparison of plant resource.Methods:In this method,C1 8column(4.6mm?250 mm)was used with the mobile phase containing acetonitrile-water for gradient elution,acetonitrile(A):0-15min,75%;15-20min,75%-95%;20-35min,95%;35-40min,95%-75%;40-50min,75%,flow rate 1 mL/min and wave length 220nm.Results:The HPLC fingerprints of Rhizoma Atractylodis macrocephalae from different origins were established and the correlated coefficients of each were calculated.9 common peaks were determined in HPLC chromatogram,three of them were identified as atractylenolideⅢ,Ⅰand atractylon respectively.Conclusion:The method was simple,reproducible and can be used as plant resource selection and quality control of Rhizoma Atractylodis macrocephalae.
