Mechanisms of inhibition on human pancreatic cancer PC-3 cell line by a diterpenoid compound 5 F isolated from Pteris semi pinnata L treatment
- VernacularTitle:半边旗中二萜类化合物5F诱导人胰腺癌细胞凋亡机制的探讨
- Author:
Bin YANG
;
Xusen LIU
;
Quanheng YUAN
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
Pteris semi pinnata L;
Diterpenoid;
Apoptosis;
PUMA
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of 5F on proliferation and apoptosis of human pancreatic cancer.Methods:The pcDNA3.1-PUMAAS and pcDNA3.1 were transfected into AsPC-1 cells by Lipofectamine 2000 methods,stable transfected clony was chosen through G418.AsPC-1 cells were divided into three groups:transfected pcDNA3.1-PUMAAS,transfected pcDNA3.1 and control group without transfection AsPC-1 cells.Three groups was treated with serial concentrations(8.875,37.5,142?mol/L,respectively)of 5F 10?L.MTT assay was used to observe the inhibitory actions of 5F on AsPC-1 cells.The apoptotic rate of the cells was detected by flow cytometry.And the apoptosis was assessed by Hoechst 33258 dye staining and TUNEL staining.The expression of PUMA protein was detected by Western blotting and RT-PCR.Results:The inhibitory action on cell growth was seen in AsPC-1 control cells and pcDNA3.1-cells dealing with 5F.It could also promote the occurrence of apoptosis.5F inhibited the proliferation of AsPC-1 cells in a concentration-dependent manner.The apoptosis induced by 5F was accompanied with the up-regulation of PUMA.In cells treated with 5F,the apoptosis rate decreased greatly and proliferation rate increased compared with the AsPC-1 control cells and pcDNA3.1-cells dealing with 5F.The expression of PUMA was decreased greatly comparing with pcDNA3.1-cells treated with 5F,Conclusion:5F can depress the proliferation of AsPC-1 cell in vitro,mainly through the induction of apoptosis,and it was a potential agent for pancreatic cancer chemotherapy,the mechanism was probably related to its effect on the regulation of PUMA expression.
