THE PROTECTIVE ROLE OF FOLATE ON NEURAL STEM CELLS UNDER HYPOXIA IN VITRO
- VernacularTitle:叶酸对体外缺氧神经干细胞保护作用的研究
- Author:
Jiajie LIU
;
Huan LIU
;
Xumei ZHANG
;
Yang YANG
;
Qingju YOU
;
Guowei HUANG
- Publication Type:Journal Article
- Keywords:
folate;
neural stem cells;
hypoxia;
proliferation;
apoptosis;
antioxidation
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of folate(Fol) on proliferation,apoptosis and the antioxidative capacity of the NSCs under hypoxia in vitro.Method The NSCs taken from infant rats brain were cultured by serum-free medium in vitro and divided into four group which were normal control group(NC),hypoxia group(Hyp),Hpy+Fol-D(folate deficient)group and Hpy+Fol group.Except NC group,the other groups were cultured in hypoxia condition for 6 h.The proliferation of NSCs after hypoxia damage was detected by MTT test.The NSCs were collected after being cultured 6 d and then the activity of SOD and GSH-PX was examined.The expression of activated caspase-3 was detected by Western blot.Results Compared with NC group,the proliferation of NSCs and the activity of SOD and GSH-PX decreased significantly after hypoxia,while the expression of activated caspase-3 increased0 The proliferation of NSCs and the activity of SOD and GSH-PX in Hyp group were significantly higher than Hyp+Fol-D group but much lower than Hpy+Fol group(P
