THE CORRELATION BETWEEN DUODENAL OXIDATIVE STRESS MEDIATED BY HIGH FAT DIET AND INTESTINAL CALCIUM ABSORPTION IN MICE
- VernacularTitle:高脂日粮介导小鼠十二指肠氧化应激与肠钙吸收的关系
- Author:
Ying XIAO
;
Jue CUI
;
Yonghui SHI
;
Jin SUN
;
Guowei LE
- Publication Type:Journal Article
- Keywords:
high-fat diet;
intestinal calcium absorption;
oxidative stress
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of high-fat diet(HFD) on redox states of duodenum and calcium absorption in mice,and to analyze the relation between them.Method Fifty C57BL/6 male mice were randomly assigned to five groups.The control group consumed an ordinary diet(0.6% Ca,w/w),and other four groups were fed with HFD(19.64%lard,0.6% Ca),HFD plus 0.1% lipoid acid(LA),HFD with calcium supplement(1.6%Ca) and HFD with 1.6% Ca and 0.1% LA supplement.Calcium apparent absorption was measured by mineral balance study after feeding for 8 w.Plasma and duodenum levels of ROS,SOD,CAT,MDA,GSH/GSSG,and T-AOC were measured to evaluate the antioxidant status.Results HFD induced oxidative stress of duodenum and decrease of intestinal calcium absorption in mice.There were positive correlations between calcium apparent absorption with GSH/GSSG(r=0.801,P
