Role of Foxp3 and regulatory T cells in pathogenesis of passive transferred myasthenia gravis in young mice
- VernacularTitle:Foxp3基因及调节性T细胞在重症肌无力被动转移幼鼠发病中的作用机制
- Author:
Xiuli WEI
;
Zhi HUANG
;
Xin LI
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
myasthenia gravis;
regulatory T cell;
Foxp3;
interleukin-2;
real-time fluorescent quantitative polymerase chain reaction;
mice,inbred C57BL
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of Foxp3 + CD4 + CD25 + regulatory T cells(Foxp3 + CD4 + CD25 + Tregs) and Foxp3mRNA in pathogenesis of passive transferred myasthenia gravis(PTMG) by detecting their expression in a PTMG model.Methods Sixteen C57BL/6 young female mice were divided into model group and control group.A PTMG model was established by injecting Ringer's solution containing 1.0 mg/kg mAb35 into abdominal cavity.Mice in control group were injected with Ringer's solution not containing mAb35.Levels of CD4 + CD25 + T cells and Foxp3 + CD4 + CD25 + Tregs in spleen cells were measured by flow cytometry.Expression of Foxp3 mRNA in spleen cells was detected by real-time fluorescent quantitative polymerase chain reaction(RT-FQ-PCR).Serum level of interleukin-2 and interferon-? was detected by ELISA.Results The ratio of CD4 + CD25 + T cells and CD4 + T cells was higher in model group(8.82 ? 0.74)% than in control group(9.89 ? 0.88)%(P
