Effect of hypoxia-inducible factor-1? on vascular relaxation reactivity following hemorrhagic shock in rats
- VernacularTitle:缺氧诱导因子-1?对失血性休克大鼠肠系膜上动脉血管环舒张反应性的调控作用
- Author:
Ken CHEN
;
Liangming LIU
- Publication Type:Journal Article
- Keywords:
hemorrhagic shock;
blood vessel;
hypoxia-inducible factor-1?;
relaxation reactivity
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hypoxia-inducible factor-1?(HIF-1?) on endotheliumdependent and-independent vascular relaxation reactivity following hemorrhagic shock in rats.Methods Two hundred and eight SD rats were randomly divided into normal control group,shock control group and oligomycin group.Shock control and oligomycin groups were further divided into 0,0.5,1,2,3,and 4 h groups after hemorrhagic shock.Superior mesenteric artery(SMA) was used to compare the changes in vascular response to sodium nitroprusside(SNP) and acetylcholine(Ach) with or without administration of oligomycin,a kind of specific HIF-1? blocking agent,and to determine the expression of HIF-1? mRNA by RT-PCR.Results The vascular reactivity of SMA to SNP in each shock group was significantly decreased at lower concentrations(10-9,10-8 and 10-7mol/L) of SNP and oligomycin further depressed the response of SMA to SNP.The response of SMA to Ach in shock groups was transiently decreased after shock,and then began to increase during the compensatory period of shock,reached its peak at 2 h after shock,and then decreased again.The response of SMA to Ach decreased significantly in early hemorrhagic shock(0 to 1 h,P
