Detection of periodental pathogens in atherosclerotic lesions in patients
- VernacularTitle:动脉粥样硬化斑块中牙周可疑致病微生物的检测
- Author:
Na AN
;
Xiangying OUYANG
;
Wei HAN
- Publication Type:Journal Article
- Keywords:
Periodontitis;
Atherosclerosis;
Porphyromonas gingivalis;
DNA,bacterial
- From:
Journal of Peking University(Health Sciences)
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect periodontopathic bacterial DNA in atherosclerotic vascular lesions in a group of Chinese patients by using polymerase chain reaction(PCR) techniques.Methods: Ten human specimens of atherosclerotic plaque were obtained sterilely.The sclerotic lesions were blade into fine pieces and DNA was isolated from the samples.To detect Porphyromonas gingivalis(Pg),Tannerella forsythia(Tf),Aggregatibacter actinomycetemcomitans(Aa),Prevotella intermedia(Pi),Prevotella nigre-scens(Pn),Treponema denticola(Td),Campylobacter rectus(Cr),PCR amplification of bacterial 16S ribosomal DNA(rDNA) was carried out.Results: PCR assays for bacterial 16S rDNA indicated the presence of periodontal pathogens in 3 out of 10 surgical specimens.DNA of Pg were found in 3 samples,Tf was found only in one among the 3 samples and Pn was found in another sample among the 3 samples.Conclusion: The data confirm that DNA of periodontal pathogens present in atherosclerotic plaques.Pg,Tf,Pn may play a role in the development and progression of atherosclerosis in these Chinese patients.Further studies with large size samples are needed.
