Changes of plasma ET-1,CGRP,TXA_2 and their receptor expression in lung tissue of children with congenital heart disease-associated pulmonary artery hypertension
- VernacularTitle:先天性心脏病肺动脉高压患儿血浆ET-1、CGRP、TXA_2及肺组织中其受体的变化
- Author:
Yuhong CAI
;
Guocheng SUN
;
Yunying MAO
;
Hong LI
- Publication Type:Journal Article
- Keywords:
congenital heart disease;
endothelin-1;
calcitonin gene related peptide;
thromboxane B2;
pulmonary artery hypertension
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of plasma ET-1,CGRP,TXA2 and their receptor expression in lung tissue of children with congenital heart disease-assicated pulmonary artery hypertension.Methods Plasma was collected from 60 children patients.ET-1,CGRP and TXB2 levels were measured by EIA,RIA and ELISA,respectively.Expression of their receptors in the lung tissue was detected by RT-PCR.Results The plasma ET-1 and TXB2 levels were significantly higher in CHD patients than in control children.The plasma CGRP level was significantly lower in CHD patients than in control children.Fourteen days after treatment with PGE1,the plasma ET-1 and TXB2 levels were significantly decreased with no significant changes in CGRP.The expression level of ET-1 and TXA2 receptor mRNA was significantly higher in patients with pulmonary artery hypertension than in those without pulmonary artery hypertension.However,the expression of CGRP receptor mRNA was lower in patients with pulmonary artery hypertension than in those without pulmonary artery hypertension.Conclusion Plasma ET-1,CGRP and TXA2 levels are closely related with congenital heart disease-associated pulmonary artery hypertension,and can be used as an index for its prognosis.
