RP-HPLC Method Measure the Content of Nutmeg Ether in the Nutmeg and Its Chane in Decoction Drug
- VernacularTitle:RP-HPLC法测定肉豆蔻煎剂中肉豆蔻醚的含量及变化
- Author:
Yong WANG
- Publication Type:Journal Article
- Keywords:
Nutmeg;
Nutmeg ether;
RP-HPLC
- From:
Journal of Zhejiang Chinese Medical University
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To measure the contents of Nutmeg ether,and its regularity varying with decoction time.[Method]RP-HPLC method was used.The chromatographic conditions were listed below Tigerkin-C18 column(150mm?4.6mm.5?m),amobine phase composed of metranol-0.025mol/L phosphoric acid(70∶30),a flow rate of 1mL ?min-1 and wave lengths was 282nm,column temperature was 25 ℃.[Results]In the range of 0.4?g ~2.0?g,the linear was very well between the content of Nutmeg ether and peak area.[Conclusion] The contents of Nutmeg ether in decoction reached the peak at 2.45min after Nutmeg ether was added in boiling water.
