Gene Expression of Cytokine Pathway,Jak-Stat Signal Pathway and Neuroactive Ligand-receptor Pathway in Patients with Primary Sjgren′s Syndrome.
- VernacularTitle:干燥综合征患者细胞因子途径、Jak-Stat信号途径以及神经激活受体配体途径相关基因表达的初步分析
- Author:
Fang WANG
;
Yongjing CHENG
;
Cibo HUANG
;
Yingjuan CHEN
;
Bei LAI
- Publication Type:Journal Article
- Keywords:
Sjgren′s syndrome;
Microarray;
Cytokine pathway;
Jak-Stat signal pathway;
Neuroactive ligand-receptor pathway
- From:
Journal of Medical Research
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study is to investigate cytokine pathway,Jak-Stat signal pathway,neuroactive ligand-receptor pathway gene expression pattern of peripheral blood mononuclear cell(PBMC) of primary sjgren syndrome patients.Methods The PBMC sample of 3 patients with sjgren syndrome and 3 healthy volunteers with consistent age were collected.The total RNAs was extracted from the PBMC samples,and reverse transcripted in vitro transcription(IVT),labeled with Cy5/Cy3 and hybridized on the gene chips.After scanning and data extraction with LuxScan 3.0,differentially expressed genes were analyzed with SAM method.The online tool of molecule analysis system(MAS) was used for biological knowledge mining.Results Statistical difference was calculated between the patient and control group in the following three pathways: cytokine pathway,Jak-Stat signal pathway,neuroactive ligand-receptor pathway.Among these,genes of IL-2RA,IL-10 were up-regulated and genes of PF4,GZMA were down-regulated.Conclusion Understanding of differently expressed genes should help us disclose the potential molecular mechanism underlying the development process of pathogenesis of primary Sjgren′s syndrome.And the research may provide new target therapy for SS.
