Expression of lentivirus-mediated short hairpin RNA (shRNA) targeting collagen type Ⅰ in rat mesangial cells
- VernacularTitle:慢病毒介导的Ⅰ型胶原shRNA在大鼠系膜细胞中的表达
- Author:
Yi SHAN
;
Chunhua ZHOU
- Publication Type:Journal Article
- Keywords:
collagen type I;
RNA interference;
lentivirus infections
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of lentivirus-mediated short hairpin RNA (shRNA) targeting collagen typeⅠ (Col Ⅰ) in rat mesangial cells.MethodsThe Col Ⅰ shRNA lentivirus vector was constructed with three-plasmids packaging approach which encoding human/rat homology Col Ⅰ gene.Rat mesangial cells were infected by infection enhancer (control group),the empty lentiviral vector (pSC-GFP group),and lentiviral expression vector (pSC-GFP/ColⅠ group),respectively.Infection efficiency in the three groups was detected with flow cytometry,and the expression of Col Ⅰ mRNA and protein in the three groups was respectively detected by RT-PCR and Western blotting.ResultsRat mesangial cells were efficiently infected in pSC-GFP group and pSC-GFP/Col Ⅰ group.The infection efficiency was 72.42% in pSC-GFP group and 75.42% in pSC-GFP/Col Ⅰ group.RT-PCR showed that the expression of Col Ⅰ mRNA in pSC-GFP/Col Ⅰ group was significantly lower than that in control group and pSC-GFP group (P
