Construction of the classification tree model of colorectal cancer with lymphatic metastasis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry
- VernacularTitle:应用SELDI-TOF-MS技术初步建立结直肠癌区域淋巴转移分类树模型
- Author:
Chunfang GAO
;
Naijun FAN
;
Xiuli WANG
;
Donghui LI
;
Guang ZHAO
- Publication Type:Journal Article
- Keywords:
spectrometry,mass,surface-enhanced laser desorption/ionization time-of-flight;
colorectal neoplasms,biological markers;
proteomics;
lymphatic metastasis
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search for the specific biomarkers associated with local lymphatic metastasis of colorectal cancer in serum.Methods The serum protein profile of colorectal cancer patients was determined by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS),and the peak values of proteins were identified with the matched software,and then clustered to construct the classification tree model.Seventy colorectal cancer patients with local lymphatic metastasis and 75 patients in matched age and gender without local lymphatic metastasis were assigned as a training set to construct the classification tree model,and 35 colorectal cancer patients with local lymphatic metastasis and 30 patients in matched age and gender without local lymphatic metastasis were assigned as test set to make the independent sample double-blind test.Results Forty-six distinct proteins were identified from the two groups,and the classification tree model formed by 5 proteins (M/Z:3104,3781,5867,7970 and 9290) could be used to identify the two groups with a sensitivity of 94.3% (66/70) and a specificity of 100.0% (75/75).The double-blind test challenged the model with a sensitivity of 91.4% (32/35),a specificity of 96.7% (29/30),and a positive predictive value of 97.0% (32/33),respectively.ConclusionThe constructed classification tree model may distinguish colorectal cancer patients with or without local lymphatic metastasis correctly,and show a great potential for preoperatively screening the colorectal cancer patients with or without local lymphatic metastasis.
