Application Effect of Protein A/G Affinity Chromatograph in Heapatitis C Virus Antibody Purification
- VernacularTitle:Protein A/G亲和层析在HCV抗体纯化中的应用效果
- Author:
Rong YANG
;
Zhongping XIE
;
Runxiang LONG
- Publication Type:Journal Article
- Keywords:
Protein A/G;
Affinity chromatograph;
Antibody;
Purification;
Effect evaluation;
Hepatitis C virus
- From:
Journal of Medical Research
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the factors of Protein A/G affinity column,influencing purification effect,and get the best condition to improve application effect of Protein A/G affinity column. Methods We Purified anti-HCV-IgG serum with different disposal modality,different sample input modality and different application number of affinity column before detecting and analyzing the purified samples. Results The Protein A/G affinity column had the best purified effect after using saturated ammonium sulfate to first purification,which increased the affinity column adsorption effect within 30 minutes adsorption. Conclusion Using antibody with first purification and adding the adsorption time could improve utilization rate of affinity column and prolong the service life.
