Influence of some factors on the optimal pH of ribonuclease from Cobra snake venom
- Author:
Nguyen Van Thiet
- Publication Type:Journal Article
- Keywords:
Cobra snake venom
- MeSH:
Cobra;
Hydrogen-Ion Concentration;
Ribonucleases
- From:Journal of Medicinal Materials - Hanoi
2005;10(5):153-158
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Evaluate the influences of some factors as enzyme level, the time of diluting enzyme solution, substrate level and temperature on pH value of RNase from Vietnam Cobra (Naja naja) venom. Results: the changes of pH value for cobra venom RNase can be depended on concentrations of the enzyme and substrate. This variation in the pH value of the enzyme can be explained by existence of this RNase as an enzyme system composed of some interconvertible forms. The interconversion between these enzyme forms is very slow process (counting by hours) in comparison with the rate of reactions
