Effect of Rhizoma Coptidis apozem on expression of AMP-activated protein kinase in skeletal muscle of metabolic syndrome rats
- VernacularTitle:黄连煎剂对代谢综合征大鼠模型骨骼肌AMPK表达的影响
- Author:
Linlin QIAO
;
Fei HUANG
;
Xiaoguang YAN
;
Huang GONG
;
Yi LI
- Publication Type:Journal Article
- Keywords:
Rhizoma Coptidis apozem;
Metabriolic syndome;
Rats;
Euglycemic hyperinsulinemic glucose clamp(GC) tecchnique;
AMPK
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of Rhizoma Coptidis apozem on expression of AMP-activated protein kinase(AMPK) in skeletal muscle of metabolic syndrome rats. Methods:The models were established by administering high fat diet. Rats were randomly divided into five group: normal control group, metabriolic syndome group, Rhizoma Coptidis apozem group, Berberine group, Metformin group. To estimate insulin resistance by euglycemic hyperinsulinemic glucose clamp(GC) tecchnique. To estimate expression of AMPK by using Western blot. Results: Compared with metabolic syndrome group, Rhizoma Coptidis apozem group had higher M-value, lower wet weight of innards fat, and higher protein level of p-AMPK-?. Conclusion: Rhizoma Coptidis apozem can improve insulin resistance, decrease innards fat, and regulate up expression of AMPK.
