Effects of colon carcinoma cells on stimulating canalization of human lymph endothelial cells in vitro
- VernacularTitle:结肠癌细胞促人淋巴管内皮细胞体外成管作用的研究
- Author:
Juanjuan OU
;
Heng JIANG
;
Rina SA
;
Houjie LIANG
- Publication Type:Journal Article
- Keywords:
endothelium,lymphatic;
colonic neoplasms;
lymphangiogenesis;
microfilaments;
microtubules;
integrin alpha9
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of colon carcinoma cells on stimulating canalization of human lymphatic endothelial cells(hLECs)in vitro.Methods hLECs in experiment group were cultured with the supernatant of colon carcinoma cell SW480,and they were cultured in endothelial culture medium in control group.The difference of the 2 groups in the ability of canalization was observed,the changes in cytoskeleton and the expression of Prox1 were detected by immunofluorescence assay,and the expression of integrin ?9 was determined by Western blotting.Results In comparison to the control group,hLECs in experiment group showed stronger ability of canalization,as a copious net-structure appeared on day 7 of cultivation,and the typical tube-structure formed finally on day 14.The number of tube-structure,including lymphatic branches,were greater in hLECs of experiment group(2.93?0.56)than control group(1.56?0.26)from day 6 on(P
