STUDY ON THE INFLUENCE OF CALCIUM, PHOSPHORUS ON PROLIFERATION, DIFFERENTIATION AND MINERALIZATION OF OSTEOBLASTS IN VITRO
- VernacularTitle:钙磷对体外培养成骨细胞增殖、分化和矿化影响的研究
- Author:
Liling ZHUO
;
Zongping LIU
- Publication Type:Journal Article
- Keywords:
SD rat;
osteoblast;
proliferation;
mineralization;
calcium;
phosphorus
- From:
Acta Nutrimenta Sinica
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the influence of Ca and P on bone metabolism by investigating theeir effects on proliferation, differentiation and mineralization of osteoblasts (OBs) in vitro. Method OBs were cultured in media with Ca(1, 2,4 mmol/L),P(1, 2,4 mmol/L),Ca:P 1:2 (1 mmol/L Ca+2 mmol/L P), and Ca:P 2:1 (2 mmol/L Ca+1 mmol/L P). Cell proliferation, activity of alkaline phosphatase (ALP), content of osteopontin (OPN) and collagen type I (Col-I) of OBs, and their corresponding mRNA expression were measured. Results Compared with control group,Ca at 4 mmol/L promoted OBs proliferation significanty (P
