Research the expression of NF-?B and apoptosis of neutrophils induced by TNF-? treated with Lidocaine
- VernacularTitle:利多卡因对中性粒细胞NF-?B的表达和TNF-?诱导的中性粒细胞凋亡的离体研究
- Author:
Nan DONG
;
Yan ZHANG
- Publication Type:Journal Article
- Keywords:
lidocaine;
anti-inflammatory;
NF-?B;
subunit p65;
apoptosis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of lidocaine to the activation of NF-?B and apoptosis in human neutrophils(PMN)in vitro.METHODS:Human PMN were divided into five groups:control group(0.9% sodium chloride);TNF-?(200 ng/L);TNF-?+lidocaine(1.0 mmol/L);TNF-?+lidocaine(2.0 mmol/L),and TNF-?+ lidocaine(4.0 mmol/L).The expression of NF-?B subunit p65 mRNA in the nuclear and I-?B mRNA in the cytosol extracts were analyzed by Western blotting,after co-incubation for 3 h and the content of p65 protein were observed by RT-PCR.PMN apoptosis was detected on flow cytometry after co-incubation 12 h and 24 h.RESULTS:The expression of NF-?B mRNA was significantly decreased and the expression of I-?B mRNA was markedly increased in lidocaine groups(P
