The Effects of Compound Zhe Bei Granule(CZBG)Combined with Doxorubicin on the Expression of GST and Topo-Ⅱ in K562/A02 Cell Line Multidrug Resistance Tumor Xenografts in Mice
- VernacularTitle:复方浙贝颗粒联合阿霉素影响K562/A02移植瘤细胞耐药相关酶表达研究
- Author:
Zhi ZHENG
;
Li HOU
;
Yamei XU
;
Dongyun LI
;
Xinyi CHEN
- Publication Type:Journal Article
- Keywords:
Compound Zhe Bei granule;
Multidrug resistance;
K562/A02 cell line;
Tumor xenograft
- From:
Journal of Medical Research
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Compound Zhe Bei granule (CZBG) combined with doxorubicin on the expression of GST and Topo - Ⅱ in K562/A02 cell line multidrug resistance tumor xenografts in mice. Methods Tumor xenografts model was established by injecting the multidrug resistance cell line K562/A02 in the axillary flank of BALB/c - nu - nu mice. Drug - comgbination of CZBG intragastric administration and doxorubicin intraperitoneal injection ( i. p. ) was given to the BALB/c - nu nude mice. The tumor xenografts were made into slice after the dissection, and the expression of GST and Topo - Ⅱ in K562/A02 tumor xenografts in mice was investigated by immunohistochemical technique. The integral optical density (IOD) of GST and Topo- lⅡ in K562/A02 tumor xenografts was measured by Image ProPlus 6.0. Results Compared with the single treatment of doxorubicin i. p group,the combination of the doxorubicin and CZBG with dosage classified by three types( high, middle, low) can decrease IOD of GSH and Topo - Ⅱ in K562/A02 tumor xenografts with statistical significance( P
