High dose thrombin suppresses proliferation and induces apoptosis of human hepatocellular carcinoma cell line HepG_2
- VernacularTitle:凝血酶对人肝癌细胞株HepG_2增殖影响及诱导凋亡作用研究
- Author:
Jun DING
;
Zhongxiong ZHUO
;
Xia ZHANG
;
Li ZHOU
- Publication Type:Journal Article
- Keywords:
thrombin;
hepatic carcinoma;
cell culture;
apoptosis
- From:Journal of Third Military Medical University
2003;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
100 U/ml and treated for 48 h,the inhibitory ratio of the HepG2 cells was raised to (39.08?2.32)%,which were not further elevated with the increase of dose or elapse of treatment time. HepG2 cells apoptosis was induced by thrombin.in a dose-and time-dependent manner when the dose was between 25 to 100 U/ml. However,there was no significant difference in the apoptosis of different groups when the dose was higher than 100 U/ml and the time exceed 48 h. Conclusion Higher doses of thrombin can inhibit the proliferation of HepG2 cells,and lead to cell apoptosis. The both effects are increased in a dose-and time-dependent manner when the dose is
