Effect of uremic serum on endothelial cell apoptosis and intervention of recombinant human erythropoietin in the process
- VernacularTitle:尿毒症患者血清对内皮细胞凋亡的影响及rhEPO的干预作用
- Author:
Xiaoli LI
;
Hua GAN
;
Xiaodong CHANG
;
Xiaogang DU
- Publication Type:Journal Article
- Keywords:
uremia;
cell apoptosis;
recombinant human erythropoietin;
oxidative stress
- From:Journal of Third Military Medical University
2003;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of uremic serum on apoptosis in human umbilical vein endothelial cells(HUVECs) and the intervention by recombinant human erythropoietin(rhEPO) in this process.Methods From Dec.2008 to Apr.2009,10 uremic patients and 10 healthy volunteers were enrolled in this study.HUVECs were divided into 3 groups:control group(including 10% healthy serum medium),uremic group(including 10% uremic serum medium) and rhEPO treatment group(rhEPO at 5,10 or 15 U/ml was added to 10% uremic serum medium).After 24 hous's intervention,cell apoptosis was evaluated by TUNEL assay,and reactive oxygen species were detected by colorimetry.Results HUVECs apoptosis index and ROS level were higher in the presence of uremic serum than those of healthy serum(P
