Effect of Semen Sojae Preparatum isoflavone on JAK2/STAT3 signal transduction pathway in proliferated vascular smooth muscle cell
- VernacularTitle:淡豆豉异黄酮对增殖血管平滑肌细胞JAK2/STAT3信号转导通路的影响
- Author:
Liying NIU
;
Yanqing REN
;
Xinguo WANG
- Publication Type:Journal Article
- Keywords:
Semen Sojae Preparatum;
isoflavone;
vascular smooth muscle cell;
proliferation;
Angiotensin Ⅱ;
Angiotensin Ⅱ receptor 1;
JAK2/STAT3 signal transduction pathway
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of Semen Sojae Preparatum isoflavone(SSPI)on proliferation and JAK2/STAT3 signal transduction pathway in rat vascular smooth muscle cell(VSMC)induced by Angiotensin Ⅱ(AngⅡ).Methods A cell proliferating model of VSMC induced by AngⅡ was established.The proliferation activity of VSMC was analyzed by MTT method.The expressions of angiotensin Ⅱ receptor 1(AT1R) were detected by RT-PCR method.The expressions of JAK2,STAT3 and phosphorylation protein were detected by Western blot.Results 100 ?g?L-1, 200 ?g?L-1 SSPI significantly inhibited the proliferation of VSMC induced by AngⅡ,and down-regulated the mRNA expression of AT1R.200 ?g?L-1 SSPI could significantly down-regulate the protein expressions of p-JAK2,p-STAT3.Conclusions The proliferation of VSMC induced by AngⅡcan be inhibited by SSPI.The mechanisms might be related to down-regulating the expressions of AT1R,and arresting the phosphorylation of JAK2/STAT3 signal transduction pathway.
