Curcumin inhibits the activity of GSK-3? to prevent AD in vitro
- VernacularTitle:姜黄素通过抑制GSK-3?的活性防治AD的体外研究
- Author:
Xiong ZHANG
;
Yu LI
- Publication Type:Journal Article
- Keywords:
Alzheimer’s disease;
curcumin;
GSK-3?;
?-catenin;
CyclinD1;
A?
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the mechanisms of Curcumin which plays a role of protecting cells through regulating the activity of GSK-3?.Methods Plasmid APPswe and BACE1-mychis were transiently co-transfected into SHSY5Y cells.The cells were treated with Curcumin and then were collected to determine the mRNA and protein levels of GSK-3?,?-catenin and CyclinD1 by RT-PCR and Western blot. Immuno-fluoresecnt staining was used to detect the location and expression of ?-catenin and GSK-3?, and ELISA was for the generation of A?.Results RT-PCR and Western blot results showed that in the cells, Curcumin inhibited the expression of GSK-3? mRNA and protein, and promoted the expression of GSK-3?-Ser9 protein (P
