Reversal multi-drug resistance by reducing the expression of CK8 and BCRP
- VernacularTitle:降低细胞膜角蛋白8和乳腺癌耐药蛋白的表达逆转耐药性
- Author:
Xiang YUAN
;
Xin CHENG
;
Yuanfu XU
;
Yuan ZHOU
;
Xiaofeng SHAO
;
Wei LI
;
Simei REN
;
Xiuli ZHANG
;
Ming YANG
- Publication Type:Journal Article
- Keywords:
mutidrug resistance;
membrane cytokeratin 8;
target multiple;
breast cancer resistant protein (BCRP);
RNAi;
hitoxantrone
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To determine whether membrane cytokeratin 8(CK8 )and BCRP expression cooperatively contributed to multidrug resistance(MDR)in MCF-7/MX cells.Methods MCF-7/MX cells were transfected with specific anti CK8-siRNAs and anti BCRP-siRNAs via LipofectAMINE2000.The expression of CK8 and BCRP was determined using Western blot,and membrane staining was observed by laser confocal microscopy.Sensitivity to chemical drugs was examined by Sulforhodamine B method.Results The expression levels of cell surface CK8 and BCRP were obviously reduced by siRNAs,and inhibition of CK8 and BCRP expression could effectively restore the sensitivity to drugs and reverse MDR phenotype of MCF-7/MX cells.Conclusions CK8 together with BCRP may play significant roles in conferring the multifactorial MDR phenotype of MCF-7/MX cells,but may act independently via potentially different mechanisms.Combinational approaches that target multiple drug-resistance-related molecules/pathways in cancer cells may represent more efficacious strategies to overcome MDR.
