Anti-proliferation role and effect on PI3K/Akt and Erk1/2 signal pathways of a chimeric anti-erbB2 antibody(chA21)on SKBR3 cells
- VernacularTitle:抗ErbB2嵌合抗体chA21对SKBR3细胞的增殖抑制以及对PI3K/Akt和Erk1/2信号转导途径的影响
- Author:
Juanjuan ZHU
;
Bin ZHAO
;
Jing LIU
- Publication Type:Journal Article
- Keywords:
breast cancer;
SKBR3;
ErbB2;
chimeric antibody;
PI3K/Akt;
Erk1/2;
signal transduction
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the inhibitory effect of anti-ErbB2 chimeric antibody chA21 on proliferation of human malignant breast cancer cell lines SKBR3 and explore its possible mechanisms through studying its effects on ErbB2 distribution and PI3K/Akt,Erk1/2 signal transduction.Methods The inhibitory effect of chA21 on SKBR3 was assessed by MTT assay.The effect of chA21 on distribution of ErbB2 on cell plasma membrane was determined by FACS(Fluorescence-activated cell sorting)analysis;p-Akt and p-Erk1/2 were detected by Western blot.Results The inhibitory rate of chA21 on SKBR3 cells in vitro increased in a dose and time dependent manner.Furthermore,it could down-regulate the distribution of ErbB2 on plasma membrane and decrease the activation of p-Akt and p-Erk1/2.Conclusions ChA21 can inhibit the proliferation of SKBR3 cell in vitro.Down-regulation ErbB2 on cell plasma membrane resulting in decreasing the activation of p-Akt and p-Erk1/2 may be one of the mechanisms of the inhibitory effect.
