The measurement of cisplatin of loading cisplatin magnetic nanomedicine in tissues by modified atomic absorption spectrometer
- VernacularTitle:改良原子吸收光谱法测定组织中载顺铂磁性纳米药物中的顺铂含量
- Author:
Lei WANG
;
Minqiang XIE
;
Shuaijun CHEN
- Publication Type:Journal Article
- Keywords:
cis-platin;
atomic absorption spectrometry;
magnetic nanomedicine;
concentration measurement;
modification;
mouse;
tissue
- From:
Chinese Pharmacological Bulletin
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a modified method of tissue digestion and cis-platin concentration measurement in tissue by atomic absorption spectrometry.Methods Using shimadzu atomic absorption spectrometry system and under the conditions of AA-6300 atomic absorption spectrophotometer,GFA-Ex7i graphite furnace,ASC-6100 automatic sampler and platinum hollow cathode discharge lamp,the cisplatin concentration of the tissues containing loading cisplatin magnetic nanomedicine which were digested with nitric acid and hydrogen peroxide in water bath,was measured by atomic absorption spectrophotometer at 265.9 nm.Results The concentrations of cis-platin in different tissues were in the linear range of 54~283.5 ?g?L-1;all the correlation coefficient were larger than 0.999 and all the inter-day and intro-day variation coefficient were smaller than 5%.Conclusion The modified method of tissue digestion and atomic absorption spectrometry applied is of high precision and efficiency, suitable for the pharmacokinetic research on cis-platin.
