Identification and Culture of Rat AO Vascular Smooth Muscle Cells by Tissue - piece Inoculation
- VernacularTitle:组织贴块法培养大鼠主动脉平滑肌细胞及鉴定
- Author:
Huan XU
;
Xin CHEN
;
Zhibing QIU
;
Chao DUAN
- Publication Type:Journal Article
- Keywords:
Smooth muscle cell;
Cell strain;
Primary culture
- From:
Journal of Medical Research
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain a lasting and purified cell line of vascular smooth muscle cells (VSMCs) by primary culture, and to provide these test materials related research. Methods The primary and transfer culture was done by tissue - piece inoculationand trypsin digestion,respectively. The cells were frozen in liquid nitrogen,and cultured cells were identified by phase contrast microscopy and immunohistochemical SP kit. Results 85% of inoculated tissue pieces survived. More than 90% of VSMCs regrew in transfered culture. The frozen cells could recover their proliferation by several culture transfers. The VSMCs showed the typical "peak and valley" characteristics under microscopy. Immunohistochemical staining with antibody against SM - a - actin expression in these cells was positive. Conclusion The tissue - piece inoculation is a simple method for obtaining satisfactory VSMCs in short term.
