Impact on the Proliferation and Cytotoxicity of Healthy Donor' s T Cells in Mobilization with rhG-CSF
- VernacularTitle:健康供者外周血干细胞内源性动员的研究
- Author:
Biaojun SHANG
- Publication Type:Journal Article
- Keywords:
rhG-CSF cell;
Proliferation;
Cytotoxocity
- From:
Journal of Medical Research
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact on the proliferation and cytotoxicity of donor T cells during mobilization with rhG - CSF. Methods The peripheral blood mononuclear cells (PBMNC) were collected from 20 healthy donors before mobilization and on the fifth day of mobilization with rhG - CSF. After the PBMNC was activated with 500 ng/ml of CD3 monoclonal antibody and 500 ?g/ml of rhIL -2 for 96 hours,the activated T cells were collected for testing proliferation, cytotoxicity. Fas expression, perforin and Fas ligand (FasL) mRNA expression,and IFN -? concentration in the culture medium of the activated T cells, were determined by radioimmunoassay. Results The proliferation activity and cytotoxicity of T cells activated with CD3 monoclonal antibody and rhIL - 2 were reduced markedly after mobilization with rhG - CSF(P0.05). The activated T cells expressed perforin mRNA and didn' t express FasL mRNA both before and after mobilization with rhG - CSF. The concentration of IFN -? in the culture medium of the activated T cells decreased significantly after mobilization with rhG - CSF(P
