Immunocompetent cells and immune regulator improve apopotosis of mesenchymal stem cells by down-regulating heme oxygenase-1
- VernacularTitle:活化T细胞及IFN-?对间充质干细胞中血红素加氧酶-1抗凋亡作用的影响
- Author:
Ling ZHANG
;
Hai BAI
;
Cunbang WANG
;
Jianfeng OU
;
Guorong YANG
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
apoptosis;
heme oxygenase-1
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of immunocompetent cells and immune regulator on the apoptosis of human mesenchymal stem cells ( MSCs) and on mRNA expression of heme oxygenase-1. Methods MSCs were cultured by density gradient centrifugation and then identified by flow cytometry. RT-PCR was used to detect HO-1 mRNA expression and flow cytometry was used to analyze cell apoptosis after the stimulation of IFN-? and PHA-activated T cells. Results The mRNA expression of Heme oxygenase-1 was observed in MSCs and decreased after the stimulation of IFN-? and activated T cells. IFN-?,znpp-Ⅸ and combined these two caused obvious cell apoptosis in MSCs,with an apoptotic rate of ( 56. 50 ? 0. 16) % ,( 56. 85 ? 2. 27) % ,and ( 82. 53 ? 2. 65) % respectively. All of them had a significant difference compared with the normal MSCs [( 7. 56 ? 1. 43) % ,P
