Naloxone hydrochloride preconditioning suppresses expressions of aquaporin protein-4 and matrix metalloproteinase-9 in rat brain tissue around cerebral hemorrhagic focus
- VernacularTitle:纳络酮预处理对脑出血大鼠脑组织水通道蛋白4及基质金属蛋白酶9表达的影响
- Author:
Ning LU
;
Xiaoyun LIU
- Publication Type:Journal Article
- Keywords:
cerebral hemorrhage;
naloxone hydrochloride;
preconditioning;
aquaporin protein 4;
matrix metalloproteinase 9
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and mechanism of naloxone preconditioning ( NP) for rats with cerebral hemorrhage. Methods Totally 30 healthy male Sprague Dawley ( SD) rats were randomly separated into Sham operatopm group,intracerebral hemorrhage ( ICH) and NP treatment groups ( n =10) . Before the establishment of rat cerebral hemorrhage model,rats in the NP group were given an intraperitoneal injection of naloxone hydrochloride ( 2. 0 mg/kg) ,and those of ICH group received an injection of normal saline at same volume. Then,the ICH model was made by injecting 0. 4 IU collagen Ⅶ into caudate nucleus while. The brain tissue samples around cerebral hemorrhagic focus after ICH were collected. RT-PCR and Western blotting were used to detect the transcriptional level and protein level of aquaporin protein 4 ( AQP4) in the brain tissue,respectively. Meanwhile,immunohistochemistry was employed to determine the expression of matrix metalloproteinase 9 ( MMP-9) in the rat brain tissue. Results Brain water content in ICH group was larger than that in Sham group ( P
