Imaging and biodistribution of ~(99)Tc~m labeled peptide high-binding VEGF receptor 3 in nude mice with ovarian cancer
- VernacularTitle:~(99)Tc~m标记VEGFR-3高亲和融合多肽在荷人卵巢癌裸鼠体内的分布和显像研究
- Author:
Ling WANG
;
Zhiqing LIANG
;
Lifeng SHI
;
Mingfu YANG
;
Guangyua LIU
- Publication Type:Journal Article
- Keywords:
vascular endothelial growth factor receptor-3;
peptide;
99Tcm;
tumor bearing mice;
imaging
- From:Journal of Third Military Medical University
2003;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the imaging and biodistribution of 99Tcm labeled peptide ( phage-SHSWHWLPNLRHYAS) high-binding VEGF receptor 3 in nude mice with ovarian cancer. Methods We used NHS-MAG3 as the bifunctional chelating agent and synthesized the peptide above. It was completed with pre-stannous direct labeling by 99Tcm and then the labeling efficiency was determined by paper chromatography. The tumor bearing mice were injected via tail veins with radiolabeled peptide. The tumors were imaged with single photon emission computed tomography ( SPECT) . We measured and calculated the biodistribution of the radiolabeled peptide. Results The labeled rate was 95. 27% and the radiochemical purity was 96% . Under a SPECT apparatus,we observed tumor location image at 1 h post injection and the tumor images was the clearest at 3 h post injection,with the injected dose per gram of tissue ( % ID/g) of ( 30. 20 ? 6. 89) at the tumor sites. The tumor/muscle ratio was 13. 13. Conclusion Our peptide can accumulate in the tumor masses. It is hopeful to be used as an ovarian cancer targeting vector for diagnosis and therapy of the cancer.
