DNA fingerprint analysis of microflora characteristics in some intestinal diseases
- VernacularTitle:几种肠道疾病患者肠道菌群结构特征的DNA指纹图谱分析
- Author:
Zhihong WANG
;
Ying HAN
;
Jiheng WANG
;
Jing ZHANG
- Publication Type:Journal Article
- Keywords:
intestinal;
microflora;
DNA fingerprinting
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the DNA fingerprint of microflora of some intestinal diseases such as ulcerative colitis (UC),acute gastroenteritis (AG) and irritable bowel syndrome (IBS),and analyze the structural characteristics of such fingerprints. Methods Thirty seven patients with intestinal diseases,definitely diagnosed by coloscopy as UC (20 cases),IBS (6 cases) and AG (11 cases),and 11 healthy people as control were involved in the present study. The total DNA was extracted from the fecal samples,and enterobacterial repetitive intergenic consensus sequence-polymerase chain reaction (ERIC-PCR) was used to set up the DNA fingerprint of intestinal microflora. The differences existed among the fingerprint profiles of intestinal microflora were compared. Results The numbers of DNA bands were obviously less in UC patients than in IBS and AG patients and healthy subjects,implying that significant differences existed in the intestinal microflora among UC and AG patients and healthy subjects. The principal band of DNA fingerprint in 17 UC patients appeared at 0.7kb,and 2 main DNA bands existed at 0.8kb and 1.1kb in AG patients,while no principal band was found in the DNA fingerprint of the IBS patients and healthy subjects. Conclusions It is likely that a single principal microflora is presented in the intestinal tissue of UC patients,which might be responsible for the morbidity of UC.
