Effect of ?-lipoic acid in inhibiting the expression of intercellular adhesion molecule-1 in human umbilical vein endothelial cells under high glucose concentration
- VernacularTitle:?-硫辛酸对高糖作用下血管内皮细胞ICAM-1表达的影响
- Author:
Junxia ZHANG
;
Guangda XIANG
;
Huilin SUN
;
Linshuang ZHAO
;
Jie HOU
- Publication Type:Journal Article
- Keywords:
thioctic acid;
glucose;
endothelial cells;
intercellular adhesion molecule-1
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ?-lipoic acid on the expression levels of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells under high glucose concentration. Methods Human umbilical vein endothelial cells (ECV304) were randomized into control (NG) group,high glucose (HG) group and ?-lipoic acid group. Cells in the 3 groups were incubated for 48h with 5.5mmol/L glucose,30mmol/L glucose,30mmol/L glucose + ?-lipoic acid in a series of concentrations (50,100,200?mol/L),respectively. The activity of superoxide dismutase (SOD) in the supernate was determined by the method of xanthine oxidase,and the content of malondialdehyde (MDA) was measured with thiobarbituric acid as the substrate. The level of ICAM-1 in the supernate was determined by enzyme-linked immunosorbent assay (ELISA). The expressive levels of ICAM-1 mRNA were determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with the control group,the activity of SOD declined significantly and the contents of MDA and ICAM-1 increased in umbilical vein endothelial cells in HG group (P
