Effects of oleanolic acid on proliferation of SGC-7901/CDDP in vitro and its potential mechanisms
- VernacularTitle:齐墩果酸对顺铂耐药胃癌SGC-7901细胞增殖的影响及其机制研究
- Author:
Hongmei LI
;
Xueyan LI
;
Defu CAI
;
Qi ZHANG
;
Jicheng LIU
- Publication Type:Journal Article
- Keywords:
oleanolic acid;
gastric cancer;
SGC-7901;
cisplatin-resistance;
Bcl-2;
Bax
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of oleanolic acid on proliferation of SGC-7901/CDDP cells in vitro and its potential mechanisms.Methods Model of SGC-7901 cells resistant to cisplatin(SGC-7901/CDDP)was established in vitro.MTT assay was used to evaluate the viability of SGC-7901/CDDP cells in vitro.Real-time PCR was used to analyse the expression of Bcl-2 and Bax gene.Results MTT assay showed that oleanolic acid dramatically inhibited the proliferation of SGC-7901/CDDP cells in vitro,more than 62% SGC-7901/CDDP cells were inhibited when the cells were treated with 100 ?mol?L-1 oleanolic acid for 72 h;Real-time PCR showed that Bax(pro-apoptotic gene)was upregulated and Bcl-2(anti-apoptotic)was downregulated after being treated with oleanolic acid.Conclusions These results suggest that oleanolic acid inhibits proliferation of SGC-7901/CDDP cells in vitro,and its potential mechanisms may be through the upregulation of Bax and the downregulation of Bcl-2.
