The apoptosis-inducing effect of MnSOD_m on K562 cells and its molecular mechanism
- VernacularTitle:锰超氧化物岐化酶模拟化合物诱导K562细胞凋亡的机制研究
- Author:
Linlan FAN
;
Hulai WEI
;
Wei DOU
;
Weisheng LIU
;
Huifang ZHANG
- Publication Type:Journal Article
- Keywords:
mimics of MnSOD;
K562 cells;
apoptosis;
Bcl-2;
Bax;
membrane potentials;
cytochrome C;
Caspase-3
- From:
Chinese Pharmacological Bulletin
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the apoptotic effect of mimics of manganese superoxide dismutase(MnSODm)on human leukemia cell line K562 in vitro and the possible molecular mechanisms.Methods Human leukemia K562 cells were used as the target cells.The cell proliferating activity was examined by a MTT colorimetric assay,and the apoptosis of K562 cells was assessed with FITC-Annexin V and propidium iodide(PI)double staining and morphological changes.The expressions of bcl-2 and bax mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR),and flow cytometry(FCM)was employed to measure the expressions of Bcl-2 and Bax protein,mitochondrial inner membrane potential(??m),Cytochrome C(Cyt C)release and Caspase-3 activity.Results The proliferation of K562 cells was obviously inhibited by 0.5~10 mg?L-1 MnSODm(P
