STUDY ON TISSUE-SPECIFICITY OF ? -CAROTENE-15,15’-MONOOXYGENASE IN CHICKEN
- VernacularTitle:?-胡萝卜素-15,15’-加单氧酶在鸡体内的组织特异性研究
- Author:
Erling WANG
;
Feng GAO
;
Xiaoguang SONG
;
Linghao CHENG
;
Guanghong ZHOU
- Publication Type:Journal Article
- Keywords:
?-carotene-15,15’-monooxygenase;
tissues;
semi-quantitative RT-PCR
- From:
Acta Nutrimenta Sinica
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the tissue-specificity of ?-carotene-15,15’-monooxygenase(? CMOOX). Method Semi-quantitative RT-PCR and HPLC were used to study the expression of ?CMOOX mRNA and its activity in different tissues of chicken. Results ?CMOOX mRNA was expressed in tissues including heart, liver, spleen, lung, kidney, duodenum, jejunum, ileum, muscle and testis, not expressed in lung. Furthermore, it was expressed in jejunum with the highest level. ?CMOOX activity was found in every tissue, except lung, and highest in duodenum and jejunum. Conclusion The distribution of ?CMOOX in chicken has tissue specificity.
