The effect of different concentration of Qianggubao decoction extract on proliferation of osteoblastic cells cultured by high glucose in vitro
- VernacularTitle:强骨宝方对体外高糖培养成骨细胞增殖功能的影响
- Author:
Zhineng CHEN
;
Youxin SU
;
Lianzi YANG
;
Liangpu ZHENG
;
Zhengyou SUN
- Publication Type:Journal Article
- Keywords:
Qianggubao decoction;
Osteoblasts;
Cells cultured by high glucose;
Cell proliferation
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of different concentration of Qianggubao decoction extract on proliferation of osteoblastic cells cultured by high glucose in vitro. Methods: osteoblastic cells was isolated from the skull of 1-2 day newly born SD rats by means of Trypsin-collagenase digestion and identified by image analysis,V-G collagen staining,ALP staining, calcification nod staining etc. Different concentrations of Qianggubao decoction extract were added to the osteoblastic cells cultured by high glucose in vitro(final concentration:300mg/dl)and incubuted.The effects of Qianggubao decoction extract on the proliferation of osteoblasts was monitored by MTT analysis. Results:Qianggubao extract of 100, 50, 10?g/ml all promoted for osteoblastic cell proliferation, the 100?g/ml and 50?g/ml had the better effects (P
