Effect of Shugan Jiannao Tiaoyu Tablets on expression of hippocamp C-FOS and C-JUN protein in post-stroke depression rat
- VernacularTitle:舒肝健脑调郁片对卒中后抑郁大鼠模型脑组织海马区C-FOS和C-JUN蛋白表达的影响
- Author:
Erping XU
;
Jinsheng ZHANG
- Publication Type:Journal Article
- Keywords:
Shugan Jiannao Tiaoyu Tablets(SJTT);
Model rats with post-stroke depression;
C-FOS/C-JUN protein
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the anti-depression mechanism of Shugan Jiannao Tiaoyu Tablets(SJTT) and provide evidene for clinical application. Methods: Depressive rat model were established by unpredictable stress with raising alone. Model rats were randomly divided into 5 groups, each 12, then each group was administrated through intragastric perfusion daily with the rate of 1.0ml/100g according to their weight before stimulation: fluoxetine group with 0.75 mg/kg, low-dose group of SJTT with 1.8g/kg, high-dose group of SJTT with 3.6g/kg, normal group and model group with 1.0ml/100g of isotonic Na chloride. The whole course lasted for 21 days from the first day of model making to the day of death. The pathologic character of hippocampus was observed by light microscopic examination. The change of cell ultramicrostructure was observed by transmission electron microscope. The expression of C-FOS and C-JUN were detected by immunohistochemistry. Results: SJTT could reduce the neuron damage of hippocam. Compared with model group, the gray scale of immunoreaction positve cells of C-FOS and C-JUN increased in high dose group of SJTT. Conclusion:SJTT can decrease the neuron damage induced by stress in hippacam and had anti-depression effect.
