A study of genetic toxicity of a neotype in situ polymeric injectable artificial prosthetic nucleus pulposus
- VernacularTitle:新型原位聚合注射性人工髓核假体的遗传毒性研究
- Author:
Kewen BAI
;
Dewei ZOU
;
Jigong WU
;
Xiaofei CHENG
;
Xuefeng ZHOU
;
Huasong MA
- Publication Type:Journal Article
- Keywords:
biocompatible materials;
arthroplasty;
prosthetic nucleus pulposus;
mutagenicity tests
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the genetic toxicity of a neotype in situ polymeric injectable artificial prosthetic nucleus pulposus.Methods The artificial prosthetic nucleus pulposus was soaked for preparing the leaching liquor which was used for tests of genetic toxicity.Salmonella typhimurium reverse mutation test(Ames Test),mammalian cell chromosome aberration(CA) test utilizing Chinese hamster ovary cells(CHO),and mouse micronucleus(MN) test were performed to detect the genetic toxicity of the extraction,including the effects on DNA,chromosome aberration and genetic mutation.Results The number of reverse mutation strains,from five strains of every dosage group,was all lower than half of quantum of control group,with or without the addition of S9,in the Ames Test,which assessed as negative.In CA test,no significant difference of chromosome aberration rate existed among the high,medium and low concentration group,while the CA rate of all the three groups was lower than that of the negative group(P0.05),but significantly lower than that of the positive control group(P
