Effect of curcumine on proliferation and heparanase expression in cultured blood vessel endothelium cells from ovarian cancer
- VernacularTitle:姜黄素对卵巢癌微血管内皮细胞生长及乙酰肝素酶表达的影响
- Author:
Ying YANG
;
Rongkai XIE
;
Ping YAN
;
Jing HAN
- Publication Type:Journal Article
- Keywords:
curcumine;
ovarian cancer;
blood vessel endothelium cell;
heparanase
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of curcumine on the proliferation and heparanase expression in blood vessel endothelium cells isolated from ovarian cancer tissues. Methods Blood vessel endothelium cells were obtained from pathologically identified ovarian cancer tissues by primary culture. The control group was the normal blood vessel endothelium cells. The experimental groups was treated with curcumine at 2,10,50 and 250 ?g/ml for 24,48 h and 72 h respectively. The cytostasis rate and cell cycle changes of the primarily cultured cells were detected by MTT assay and flow cytometry respectively. The transcriptional and translation levels of heparanase were detected by RT-PCR and immunofluorescent staining. The MDA content and LDH activity in the supernatant were detected too. Results In the curcumine groups,the cell proliferation was inhibited in a dose-dependent and time-dependent manner ( P
