Effects of Nordy on proliferation,cell cycle and Aurora-A expression in human ovarian cancer cell lines 3AO and SKOV3
- VernacularTitle:诺帝对人卵巢癌细胞3AO和SKOV3增殖、周期及Aurora-A表达的影响
- Author:
Shijun JIA
;
Yong ZHAO
;
Xiuwu BIAN
- Publication Type:Journal Article
- Keywords:
Nordy;
ovarian cancer;
cell cycle;
Aurora-A
- From:Journal of Third Military Medical University
2003;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Nordy on the proliferation,cell cycle,and the mRNA and protein expressions of Aurora-A in human ovarian cancer cell lines 3AO and SKOV3. Methods After being treated with Nordy at the doses of 25,50 or 100 ?mol/L,the proliferation of 3AO and SKOV3 cells were tested with MTT assay; The expression of Aurora-A was detected by RT-PCR and Western blotting; The effect on cell cycle were analyzed by flow cytometry ( FCM) . Results Treated with Nordy,the mRNA and protein level of Aurora-A gene were significantly reduced ( P
