The protective effects of chloride channel blockers on the cultured hippocampal neuronal apoptosis induced by NO in rats
- VernacularTitle:氯通道阻断剂对NO诱导离体大鼠海马神经元凋亡的保护作用
- Author:
Quanzhong CHANG
;
Shuling ZHANG
;
Jinbao YIN
;
Shining CAI
- Publication Type:Journal Article
- Keywords:
chloride channel blocker;
NO;
hippocampal neuron;
rat;
apoptosis;
Caspase-3
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the protective effects of SITS and DIDS,two kinds of chloride channel blockers,on hippocampal neuronal damage induced by NO in culture.Methods The cultures were divided into three groups:control group,NO treatment group,NO treatment plus chloride channel blocker group. The cultures were detected with the methods of morphological stain (Hoechst 33258),and the apoptotic neurons and neuronal viabilities were observed through MTT quantitative analysis. The activated caspase-3 was analyzed with western blot.Results There were significant protective effects of SITS and DIDS on neuronal damage with dose-dependence.Conclusions Chloride channel blockers have some protective effects against neuronal injury induced by NO.
