NO pathway involves in the anti-hypertrophic effects of fenofibrate induced by high glucose and insulin
- VernacularTitle:一氧化氮参与非诺贝特抗高糖高胰岛素诱导的心肌肥大
- Author:
Mingfeng WANG
;
Qingsong JIANG
- Publication Type:Journal Article
- Keywords:
peroxisome proliferator-activated receptor-?;
nitric oxide synthase;
nitric oxide;
cardiomyocyte hypertrophy;
high glucose and insulin;
fenofibrate;
diabetes mellitus
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of fenofibrate (FF),a selective PPAR-? agonist,in cardiac hypertrophy induced by high glucose and insulin (HGI) and its mechanisms related to nitric oxided (NO) signal transduction pathway. Methods The cultured neonatal rat cardiomyocytes were used to observe the effects of FF on cardiomyocyte hypertrophy induced by HGI (glucose at 25.5 mmol?L-1 and insulin at 0.1 ?mol?L-1),and the cardiomyocyte hypertrophic responses were assayed by measuring the cell surface area,protein content,and atrial natriuretic factor (ANF) mRNA expression. The expressions of mRNA and protein were assayed by Real-time PCR and Western blot,as well as NOS activity and NO concentration in cultured media were determined by using the spectrophotometry and nitrate reluction method.Results In cultured cardiomyocytes,FF (at 0.1,0.3 and 1 ?mol?L-1) could inhibit the cardiomyocyte hypertrophy induced by HGI in a concentration-dependent manner (P
